rabbit anti interferon beta (Bioss)
Structured Review
![Impact of IRF7 knockdown and H151 pre-treatment on microglial inflammatory responses and phenotypic markers in BV2 cells exposed to MPP + . (A) IRF7 mRNA expression in cells subjected to si-IRF7 knockdown was determined by RT-PCR, which showed an approximate 80% reduction ( n = 3 independent experiments). (B) BV2 cells were pre-treated with si-IRF7 for 60 hours and then exposed to MPP + for an additional 24 hours. Western blot analysis was used to detect the relative protein levels of IRF7 and IFN-β in BV2 cells stimulated with MPP + after si-IRF7 pre-treatment ( n = 5 independent experiments). (C) Western blotting was used to detect the relative protein levels of iNOS and TNF-α in BV2 cells stimulated with MPP + after si-IRF7 pre-treatment ( n = 5 independent experiments). (D) BV2 cells were pre-treated with H151 (5 µM) for 12 hours and then exposed to MPP + for another 24 hours. RT-PCR was used to detect markers of the M1 ( CD16 , CD32 , and CD86 ) and M2 ( ARG1 , YM1 ) phenotypes ( n = 6 independent experiments). Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 (unpaired t -test [A] or one-way analysis of variance followed by Tukey’s post hoc test [B–D]). ARG1: Arginase 1; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; H-151: a STING inhibitor; IFN-β: <t>interferon-beta;</t> IRF7: interferon regulatory factor 7; iNOS: inducible nitric oxide synthase; MPP+: 1-methyl-4-phenylpyridinium; ns: not significant; RT-PCR: real-time polymerase chain reaction; si-IRF7: small interfering RNA targeting IRF7; TNF-α: tumor necrosis factor-alpha.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_9022/pmc11759022/pmc11759022__NRR-20-2361-g006.jpg)
Rabbit Anti Interferon Beta, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti interferon beta/product/Bioss
Average 94 stars, based on 5 article reviews
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1) Product Images from "The cGAS-STING-interferon regulatory factor 7 pathway regulates neuroinflammation in Parkinson’s disease"
Article Title: The cGAS-STING-interferon regulatory factor 7 pathway regulates neuroinflammation in Parkinson’s disease
Journal: Neural Regeneration Research
doi: 10.4103/NRR.NRR-D-23-01684
Figure Legend Snippet: Impact of IRF7 knockdown and H151 pre-treatment on microglial inflammatory responses and phenotypic markers in BV2 cells exposed to MPP + . (A) IRF7 mRNA expression in cells subjected to si-IRF7 knockdown was determined by RT-PCR, which showed an approximate 80% reduction ( n = 3 independent experiments). (B) BV2 cells were pre-treated with si-IRF7 for 60 hours and then exposed to MPP + for an additional 24 hours. Western blot analysis was used to detect the relative protein levels of IRF7 and IFN-β in BV2 cells stimulated with MPP + after si-IRF7 pre-treatment ( n = 5 independent experiments). (C) Western blotting was used to detect the relative protein levels of iNOS and TNF-α in BV2 cells stimulated with MPP + after si-IRF7 pre-treatment ( n = 5 independent experiments). (D) BV2 cells were pre-treated with H151 (5 µM) for 12 hours and then exposed to MPP + for another 24 hours. RT-PCR was used to detect markers of the M1 ( CD16 , CD32 , and CD86 ) and M2 ( ARG1 , YM1 ) phenotypes ( n = 6 independent experiments). Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 (unpaired t -test [A] or one-way analysis of variance followed by Tukey’s post hoc test [B–D]). ARG1: Arginase 1; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; H-151: a STING inhibitor; IFN-β: interferon-beta; IRF7: interferon regulatory factor 7; iNOS: inducible nitric oxide synthase; MPP+: 1-methyl-4-phenylpyridinium; ns: not significant; RT-PCR: real-time polymerase chain reaction; si-IRF7: small interfering RNA targeting IRF7; TNF-α: tumor necrosis factor-alpha.
Techniques Used: Knockdown, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Real-time Polymerase Chain Reaction, Small Interfering RNA